transgenic cattle pdf

0000011585 00000 n 0000005102 00000 n 0000002392 00000 n 3 0 obj However, this procedure is characterized by low efficiency (1-4% transgenic offspring), random integration and variable expression of the transgene as well as a considerable proportion of mosaicism. Furthermore, it is extremely time consuming and costly. Academia.edu is a platform for academics to share research papers. 0000009994 00000 n 0000054631 00000 n 0000007329 00000 n 0000008376 00000 n )�@�BT�,՛��A�.Q��zw� 0000011885 00000 n endobj 0000020288 00000 n Microinjection of DNA constructs into pronuclei of zygotes has been the method of choice for the generation of transgenic livestock. 0000008851 00000 n 0000008091 00000 n Gene transfer is a beneﬁcial way of modifying the genome of domestic livestock. y�x��'�H�& 9�X��A��v|}�)��!hg�θ�#��l�K�ψ�� %�� 0000009802 00000 n % B��@}pQa��0��III ,��. 0000029127 00000 n m{�W$�Ԫt}ha��@P�CoMj�< 0000007426 00000 n The transgenic cattle line TG3 used in this study has been modiﬁed with additional gene copies for the milk proteins b-andk-casein and was previously shown to express the transgenes at high levels [18]. Transgenic mice generated by pronuclear injection of a yeast artificial chromosome. 0000006741 00000 n O��iFv�h:��56�^��wH�[�_j�&T$. } 0000002657 00000 n 0000004024 00000 n Developments in transgenic techniques in pigs. Furthermore, the develop-ment of transgenic farm animals will enable greater ﬂexi-bility in direct genetic manipulation of livestock. ��D8$�8��!��$��9�i�|f_�]��5��@84�}�G��bb�W��?p��ޚ]&��&V�.-Jmf��+��l�� 'f�ڶ�Iy��6��KG+�{��l"��얓ݸ��z�ٜ��oK�Fs 0000053223 00000 n Efficient production of transgenic cattle by retroviral infection of early embryos, By clicking accept or continuing to use the site, you agree to the terms outlined in our. 0000018570 00000 n Some features of the site may not work correctly. ��¡��~�{�v!�m��3߉�pC#ot�µ��כ��^��C��כ�s�W}��с��M#@F0S��! trailer << /Size 341 /Info 238 0 R /Root 240 0 R /Prev 402718 /ID[<1b1858040fd561c599203b1745ae1faa><1b1858040fd561c599203b1745ae1faa>] >> startxref 0 %%EOF 240 0 obj << /PageMode /UseOutlines /Names 243 0 R /Outlines 245 0 R /Metadata 237 0 R /Pages 234 0 R /PageLayout /SinglePage /OpenAction [ 244 0 R /FitH 910 ] /Threads 241 0 R /Type /Catalog /PageLabels 232 0 R >> endobj 241 0 obj [ 242 0 R ] endobj 242 0 obj << /F 270 0 R /I << /Title (Main_Text)>> >> endobj 243 0 obj << /Dests 144 0 R >> endobj 339 0 obj << /S 464 /T 709 /O 765 /E 781 /L 797 /Filter /FlateDecode /Length 340 0 R >> stream J�� V0Eb��d9��\��AE��z��0TP�}b��&.��4@[.��&5`c��8�oG6A transgenic cattle will remain a severe limitation to realizing the potential of transgenic cattle until ineffi-ciencies of transgenic technology are overcome. 0000003957 00000 n 0000009231 00000 n 0000004474 00000 n 0000002722 00000 n 0000017798 00000 n 0000005584 00000 n 0000004379 00000 n 0000018592 00000 n 0000015412 00000 n 0000009898 00000 n %PDF-1.4 %�� 0000009137 00000 n �Ջ�#��9A^�R�?s��aNpʩe�*�u��t�U��/.+1�*��V2NJ�w�heB��4U �T��3��1��*�#V��&� TفW��9z��%��\��Pd'�r �bЦH�˙�HA]O�:�Y�. As a consequence, commercial application has focused on the production of recombinant proteins in the mammary…, Precision genetic modification of the Mammalian Genome, Following Intracytoplasmic Sperm Injection, Protein Expression Using Transgenic Animals, Espermatozóide bovino como vetor de transgene: interação do DNA exógeno e viabilidade espermática, Cell-based systems as an alternative to animal models, Biotecnologías de reproducción animal con posibilidad de aplicación para optimizar el potencial reproductivo y productivo de los animales. 0000009610 00000 n Genetic modification of animals in the next century. 0000016920 00000 n 0000020848 00000 n 0000006610 00000 n 0000016071 00000 n 0000008186 00000 n 0000017776 00000 n 0000016049 00000 n 0000004222 00000 n 0000021330 00000 n 0000005692 00000 n You are currently offline. stream 0000009325 00000 n 0000009418 00000 n tion and maintenance of transgenic mammals such as sheep, cattle, and pigs involves a large investment in time and resources, which prevents their use in most research set-tings. pH ��C!��R)� �qˈ�\5��o�L�PR2�̦��U��N�ʤ�m3=9��l.�P��h�\�9+*�J��#�8�3��:�*�О/��&��?O��1HJ�� Rf�9hÜ�9�*��)�X�)p8P!��2�â��2Hc��H��d�ጨr8` 0000005354 00000 n 0000021649 00000 n The use of these methodologies will YAC transgenesis in farm animals: Rescue of albinism in rabbits, Transgenic technology in animal agriculture. Furthermore, it is extremely time consuming and costly. 0000007113 00000 n /Filter /LZWDecode f��F0��ي��R��;�v�#-�o�ln�i�I c�: �,�C?tc|�,�|#ȃ(�=�P"�u��/_z�}�\��; ��W+3-A�0�I°!�V��m&B��'�W&�B��& ��i5��X�) However, this procedure is characterized by low efficiency (1-4% transgenic offspring), random integration and variable expression of the transgene as well as a considerable proportion of mosaicism. 0000006918 00000 n 0000007902 00000 n 1 0 obj 0000009042 00000 n �,�4��:�$#`� ��P"��*: �$��P7�ʪ�#p�6̪�� R�!��B0�7��pj�+R#�4=��2��?xP+K�h�"ӊ�� H�3��Ы�A��TMX�C��ls+N�IiѻX�;��@�{� HS�p��L�&��;cx�:��0�I�P#B�mt3ު��9t4��z��?��[��&��7�� '5 0000015784 00000 n 0000029831 00000 n 0000003535 00000 n 0000010403 00000 n 0000008662 00000 n 0000006340 00000 n 0000007713 00000 n H�b```e`�:�� Ā Bl@Q�-2 0000010186 00000 n 0000010090 00000 n These inefficiencies include low rates of gene integration, poor embryo survival, and unpredictable transgene behavior. 0000006075 00000 n 0000003557 00000 n Microinjection of DNA constructs into pronuclei of zygotes has been the method of choice for the generation of transgenic livestock. Transgenic Cattle: Cattle is a combined term used for oxes, bulls, buffalos, cows, etc. 0000008947 00000 n [/CalRGB 0000007808 00000 n 0000005808 00000 n 0000020266 00000 n 0000013509 00000 n >> 0000016898 00000 n 0000019401 00000 n 0000006475 00000 n 0000007233 00000 n As such the use of 0000009706 00000 n 0000004318 00000 n 0000005465 00000 n 0000008567 00000 n 0000019423 00000 n 0000009514 00000 n 0000021236 00000 n 0000014508 00000 n Transgenic cattle resulting from biopsied embryos: expression of c-ski in a transgenic calf. 0000014530 00000 n 0000007618 00000 n cations of transgenic methodology to develop new and improved strains of livestock. %PDF-1.1 0000007522 00000 n 0000013487 00000 n 0000004851 00000 n 0000012688 00000 n >> 0000004594 00000 n Resource requirements for transgenic livestock research. Cloned transgenic cattle from line TG3 and control cows (WT) produced by nuclear transfer from the 0000011169 00000 n 0000008471 00000 n 0000008757 00000 n 0000002626 00000 n Title: Transgenic milk Author: Administrator Created Date: 3/2/2009 3:03:34 PM �R��j��7�\2��z�n�)V'�["��f��0��d��7��C#l ��JŲ�CNe-�T��0r ᴜ'W?�d�m�X�$�� 5)�YH��ʘ��5 ��TGx3ˠ� �6��KN'>��E 0t9�� 0000005263 00000 n Production and breeding of transgenic cattle using in vitro embryo production technology. 0000032045 00000 n 0000012488 00000 n Cattle are farmed for beef, veal, dairy, leather, etc. �S��hL�Bvx��ؙ�I;�@7��I�v+!�M*CtU��C`ta�05�8OhDf`ć�PhsX��QFH�"Ģ��%ϑܘW�M��q��߁�!

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